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rrna depletion  (New England Biolabs)


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    New England Biolabs rrna depletion
    Rrna Depletion, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 181 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e7405/pmc13122172-60-15-17?v=New+England+Biolabs
    Average 96 stars, based on 181 article reviews
    rrna depletion - by Bioz Stars, 2026-07
    96/100 stars

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    (A) Diagram of sucrose gradient fractions used for heavy polysome analysis. (B) qPCR of candidate transcripts in the heavy polysome fraction (≥3 ribosomes per transcript), normalized to 28S <t>rRNA</t> (n = 3; mean ± SD). (C) Schematic of RPA calculation. Polysome association (PA = Heavy/Input) is computed per condition; RPA = PA dTAG /PA DMSO , reported as log₂(RPA). Heatmap shows gene classification across replicates. (D) Correlation between qPCR fold change and RNA-seq–based log₂RPA for 13 validated transcripts spanning the sensitivity spectrum (n = 3; mean ± SD).
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    (A) Diagram of sucrose gradient fractions used for heavy polysome analysis. (B) qPCR of candidate transcripts in the heavy polysome fraction (≥3 ribosomes per transcript), normalized to 28S rRNA (n = 3; mean ± SD). (C) Schematic of RPA calculation. Polysome association (PA = Heavy/Input) is computed per condition; RPA = PA dTAG /PA DMSO , reported as log₂(RPA). Heatmap shows gene classification across replicates. (D) Correlation between qPCR fold change and RNA-seq–based log₂RPA for 13 validated transcripts spanning the sensitivity spectrum (n = 3; mean ± SD).

    Journal: bioRxiv

    Article Title: Acute EIF4G1 depletion reveals transcript-wide determinants of initiation factor dependence and activates initiation-specific ribosome quality control

    doi: 10.64898/2026.04.17.719311

    Figure Lengend Snippet: (A) Diagram of sucrose gradient fractions used for heavy polysome analysis. (B) qPCR of candidate transcripts in the heavy polysome fraction (≥3 ribosomes per transcript), normalized to 28S rRNA (n = 3; mean ± SD). (C) Schematic of RPA calculation. Polysome association (PA = Heavy/Input) is computed per condition; RPA = PA dTAG /PA DMSO , reported as log₂(RPA). Heatmap shows gene classification across replicates. (D) Correlation between qPCR fold change and RNA-seq–based log₂RPA for 13 validated transcripts spanning the sensitivity spectrum (n = 3; mean ± SD).

    Article Snippet: Libraries were prepared using the NEBNext rRNA Depletion Kit v2 (NEB E7405) followed by the Illumina TruSeq RNA Sample kit.

    Techniques: RNA Sequencing